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The Role of Pcdh10a and Pcdh10b in Zebrafish Melanocyte Migration
Başlık:
The Role of Pcdh10a and Pcdh10b in Zebrafish Melanocyte Migration
Yazar:
Williams, Jason S., author.
ISBN:
9780438003057
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (91 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Includes supplementary digital materials.
Advisors: Kristin Artinger; Chad Pearson Committee members: Linda Barlow; David Clouthier; Heide Ford; Lee Niswander.
Özet:
Proper cell migration during embryonic development is crucial for appropriate tissue patterning and the final vertebrate body plan. A highly migratory cell type during development that is unique to vertebrates is the neural crest. Neural crest cells arise on the dorsal side of the embryo and migrate to nearly every part of the developing embryo. These migratory cells will go on to form several different tissue types including the neurons and glia of the peripheral nervous system, craniofacial cartilage and bones, and melanocytes. To control this dynamic migration, several factors are needed to regulate cell adhesion at sites of cell-cell contact and interactions with other neural crest cells and the environment, as well as factors that act as guidance and signaling molecules. The goal of this thesis is to examine the role of Pcdh10a and Pcdh10b in the migration of neural crest-derived melanocytes. Here, I have demonstrated that pcdh10a is expressed in melanocytes and that the protein localizes to the membrane in the expressing cells. When pcdh10a expression is reduced, a small population of melanocytes fail to migrate to the lateral stripe and instead differentiates inside the embryo. Live cell imaging analysis demonstrates that loss of pchd10a results in a reduction of directed cell migration of melanocyte precursors, caused by both increased adhesion and a loss of cell-cell contact with other migratory neural crest cells. I identified that in the pcdh10a genetic mutant, the paralog pcdh10b is being upregulated to compensate for the loss of pcdh10a, and when both paralogs are removed, this is lethal to the developing embryo. Taken together, this work demonstrates the importance of Pcdh10a in the migration of neural crest-derived melanocytes, as well as how organisms adapt to changes in their genetic material.
Notlar:
School code: 1639
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(680525.1) | 680525-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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