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Green fluorescent proteins
Başlık:
Green fluorescent proteins
Yazar:
Sullivan, Kevin Francis, 1955-
ISBN:
9780080859569
9780125441605
9781281755568
9786611755560
Yayın Bilgileri:
San Diego, Calif. ; London : Academic Press, ©1999.
Fiziksel Tanımlama:
1 online resource (xvii, 386 pages, 18 unnumbered pages of plates) : illustrations (some color).
Seri:
Methods in cell biology, v. 58
Methods in cell biology ; v. 58.
Genel Not:
"Prepared under the auspices of the American Society for Cell Biology."
İçerik:
Biophysics of the green fluorescent protein / Understanding structure-function relationships in the Aequorea victoria green fluorescent protein / Quantitative imaging of the green fluorescent protein (GFP) / Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics / Targeting GFP to organelles / Cytoskeletal dynamics in yeast / Analysis of nuclear transport in vivo / GFP fusion proteins as probes for cytology in fission yeast / GFP variants for multispectral imaging of living cells / GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila / GFP as a cell and developmental marker in the Drosophila nervous system / Using time-lapse confocal microscopy for analysis of centromere dynamics in human cells / Visualization of large-scale chromatin structure and dynamics using the lac operator/lac repressor reporter system / Centrosome dynamics in living cells / Transfections of primary muscle cell cultures with plasmids coding for GFP linked to full-length and truncated muscle proteins / Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein / Synchronous real-time reporting of multiple cellular events / Visualizing protein interactions in living cells using digitized GFP imaging and FRET microscopy / Flow cytometric analysis and FACS sorting of cells based on GFP accumulation / GFP biofluorescence : imaging gene expression and protein dynamics in living cells
Özet:
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features* Only volume on Green Fluorescent Protein research* Covers all aspects of GFP* Provides specific microscopic and data analysis methods* Discusses the design and construction of GFP fusion proteins* Covers GFP expression in animals, insects, plants.
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Tür:
Tüzel Kişi Ek Girişi:
Elektronik Erişim:
ScienceDirect http://www.sciencedirect.com/science/book/9780125441605 ScienceDirect http://www.sciencedirect.com/science/bookseries/0091679X/58Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
QH585 .M47 EB VOL. 58 | 1190356-1001 | Elsevier E-Kitap Koleksiyonu | Arıyor... |
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