Front Cover; Methods in Cell Biology, Volume 31; Copyright Page; Contents; Contributors; Preface; PART I: GAINING ACCESS TO THE CYTOPLASM; Chapter 1. Lysed Chromatophores: A Model System for the Study of Bidirectional Organelle Transport; I. Introduction; II. Methods to Obtain, Lyse, and Reactivate Chromatophores; III. Mechanisms of Organelle Transport in Chromatophores; IV. Summary; References; Chapter 2. Digitonin Permeabilization Procedures for the Study of Endosome Acidification and Function; I. Introduction; II. Prepartion of Mannose-BSA for Endosome Acidification Studies

III. Permeabilization of CellsIV. Endosome Acidification; V. Summary and Outlook; References; Chapter 3. ATP Permeabilization of the Plasma Membrane; I. Introduction; II. Plasma Membrane Receptors for Adenine Nucleotides; III. ATP Permeabilization; IV. Recovery of Intracellular Homeostasis following ATP Permeabilization; V. Assessment of Permeabilization; VI. ATP-Resistant Cells; VII. Uses of ATP Permeabilization; VIII. Relative Merits of ATP as a Means of Membrane Permeabilization; IX. Mechanism of ATP-Induced Permeabilization; X. Conclusion; References

Chapter 4. Poration by a-Toxin and Streptolysin 0: An Approach to Analyze Intracellular ProcessesI. Introduction; II. Purification and Analysis of Pore-Forming Toxins; III. Application for Cell Poration; IV. Pore-Forming Toxins as Tools in the Study of Intracellular Processes; V. Concluding Remarks; References; Chapter 5. Preparation of Semiintact Chinese Hamster Ovary Cells for Reconstitution of Endoplasmic Reticulum-to-Golgi transport in a Cell-Free System; I. Introduction; II. Growth of Cells; III. Preparation of Semiintact Cells from Adherent Cells

IV. Preparation of Semiintact Cells from Suspension CellsV. Incubation Conditions to Achieve Transport between the ER and Golgi in Vitro; VI. Discussion; References; Chapter 6. Perforated Cells for Studying Intracellular Membrane transport; I. Introduction; II. Generation and Characterization of Perforated Cells; III. Reconstitution of Intracellular Membrane transport; IV. Discussion; References; Chapter 7. Reconstitution of Protein Transport Using Broken Yeast Spheroplasts; I. Introduction; II. Preparation of Transport-Competent Membranes; III. Reconstitution of Protein Transport

IV. Future ProspectsReferences; Chapter 8. Reconstitution of Transport from the ER to the Golgi Complex in Yeast Using Microsomes and Permeabilized Yeast Cells; I. Introduction; II. In Vitro Translation of Prepro-a-factor in a Yeast Lysate; III. In Vitro Translocation into the ER Lumen of PYC or Microsomes; IV. In Vitro Transport of Pro-a-factor from the ER to the Golgi Complex; V. Summary; References; Chapter 9. Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein; I. Introduction; II. Development of Cell Lines that Express the Influenza HA