Purification and Biochemical Properties of Rac1, 2, 3 and the Splice Variant Rac1b -- Biochemical Analyses of the Wrch Atypical Rho Family GTPases -- Purification of P-Rex1 from Neutrophils and Nucleotide Exchange Assay -- In Vitro Guanine Nucleotide Exchange Activity of DHR-2/DOCKER/CZH2 Domains -- Biochemical Characterization of the Cool (Cloned-Out-of-Library)/Pix (Pak-Interactive Exchange Factor) Proteins -- GEF and Glucosylation Assays on Liposome-Bound Rac -- Phosphorylation of RhoGDI by p21-activated kinase 1 -- Purification of ARAP3 and Characterization of GAP Activities -- Regulation of RhoGAP specificity by Phospholipids and Prenylation -- Purification and Activity of the Rho ADP-ribosylating binary C2/C3 Toxin -- Purification of TAT-C3 Exoenzyme -- Imaging and photobleach correction of Mero-CBD, Sensor of Endogenous Cdc42 Activation -- Cdc42 and PI(4,5)P2-induced actin Assembly in Xenopus Egg Extracts -- In vitro reconstitution of Cdc42-mediated actin assembly using purified components -- Biochemical analysis of mammalian formin effects on actin Dynamics -- Formin proteins: purification and measurement of effects on actin assembly -- Purification and kinase assay of PKN -- Purification and enzyme activity of ACK1 -- Direct Activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid Vesicles -- Regulation of PLCbeta isoforms by Rac -- Biochemical properties and Inhibitors of (N- )WASP -- The Use of GFP to localize Rho GTPases in living cells -- Analysis of the spatiotemporal activation of Rho GTPases using Raichu probes -- Measurement of activity of Rho GTPases during mitosis -- Inhibition of Rho GTPases by RNA interference -- RNA interference techniques to study epithelial cell adhesion and polarity -- Nucleofection of Primary Neurons -- Dock180-ELMO cooperation in Rac activation -- Rho GTPase activation by cell-cell adhesion -- Activation of Rap1, Cdc42, and Rac by nectin adhesion system -- Analysis of activated GAPs and GEFs in cell lysates -- Degradation of RhoA by Smurf1 ubiquitin ligase -- Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin -- Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells -- Activation of MEKK1 by Rho GTPases -- Activation of the apoptotic JNK pathway through the Rac1-binding scaffold protein POSH -- Quantification of isozyme-specific activation of phospholipase C-beta2 by Rac GTPases and phospholipase C-epsiv by Rho GTPases in an intact cell assay system -- Activation of Rho and Rac by Wnt/frizzled signaling -- Fluorescent assay of cell-permeable C3 transfease activity -- Use of TIRF microscopy to visualize actin and microtubules in migrating cells -- Inhibition of ROCK by RhoE -- Conditional Regulation of a ROCK-estrogen receptor fusion protein -- Rational design and applications of a Rac GTPase-specific small molecule Inhibitor -- In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization -- Real-time centrosome reorientation during fibroblast migration -- Lentiviral delivery of RNAi in hippocampal neurons -- Methods for studying neutrophil chemotaxis -- Measurement of epidermal growth factor receptor turnover and effects of Cdc42 -- Tumor cell migration in three dimensions -- An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration -- Analysis of a mitotic role of Cdc42 -- Plexin-induced collapse assay in COS cells -- Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures -- Using three-dimensional acinar structures for molecular and cell biological assays -- TC10 and insulin-stimulated glucose transport -- GTPases and the control of neuronal polarity -- In vitro assembly of filopodia-like bundles.