Eylem Seç
Proteomic Analysis of the ade6-M26 Meiotic Recombination Hotspot in the Fission Yeast Schizosaccharomyces pombe
Başlık:
Proteomic Analysis of the ade6-M26 Meiotic Recombination Hotspot in the Fission Yeast Schizosaccharomyces pombe
Yazar:
Storey, Aaron Joseph, author.
ISBN:
9780438035799
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (252 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Wayne P. Wahls Committee members: Mari K. Davidson; Robert L. Eoff; Randy S. Haun; Alan J. Tackett.
Özet:
Homologous recombination during meiosis mediates reductional segregation of chromosomes and increases genetic diversity among progeny. Errors in meiotic recombination can result in miscarriages, congenital birth defects, and infertility. Hotspots regulate the frequency and position of recombination events during meiosis, but their mechanism of activation remains poorly understood. Current models of hotspot activation involve post-translational modifications of histones and recruitment of chromatin remodeling enzymes to the hotspot. To test these models and to identify novel regulators of meiotic recombination hotspots, an affinity-capture approach was developed and implemented to purify the ade6-M26 meiotic recombination hotspot from fission yeast and quantify proteins associated with this locus using mass spectrometry.
Small, circular minichromosomes containing the ade6-M26 hotspot or control locus were constructed and transformed into fission yeast strains and purified from synchronous meiotic cultures. Analysis of the large-scale data sets and validation experiments revealed five novel regulators of the ade6-M26 meiotic recombination hotspot. The histone chaperones Hir1 and Nap1, the INO80 complex subunits Arp5 and Arp8, and the ATP-dependent chromatin remodeling enzyme Fft3 contribute to hotspot activation through distinct, possibly overlapping mechanisms. More than 30 unique, combinatorial histone post-translational modifications were detected and quantified between hotspot and control throughout meiosis. Overall, this dissertation work demonstrates the utility of affinity purification experiments coupled to mass spectrometry to reveal novel insights into the regulation of crucial biological processes, and suggests a conserved mechanism of hotspot activation through the interplay between these protein complexes and the H2A.Z histone variant.
Notlar:
School code: 0365
Tüzel Kişi Ek Girişi:
Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(678230.1) | 678230-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
On Order
Liste seç
Bunu varsayılan liste yap.
Öğeler başarıyla eklendi
Öğeler eklenirken hata oldu. Lütfen tekrar deneyiniz.
:
Select An Item
Data usage warning: You will receive one text message for each title you selected.
Standard text messaging rates apply.