Eylem Seç
Targeting Neuroinflammation for Disease Modification in Models of Parkinsonism
Başlık:
Targeting Neuroinflammation for Disease Modification in Models of Parkinsonism
Yazar:
Hammond, Sean L., author.
ISBN:
9780438042384
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (158 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Ronald B. Tjalkens Committee members: Gerrit Bouma; Deborah Garrity; Marie Legare.
Özet:
Chronic inflammation of the central nervous system (CNS) is a large contributing factor to neurodegeneration. Neuroinflammation is mediated by cellular communication between microglia, astrocytes, neurons and invading leukocytes from the periphery in response to neuronal injury. The second most common neurodegenerative disorder, Parkinson's disease (PD), is characterized by progressive loss of dopaminergic (DA) neurons from the substantia nigra (SN) and sustained neuroinflammatory activation of glial cells in the vicinity of these neurons. One master regulator of glial cell activation is transcription factor, NF- kappaB. NF- kappaB is ubiquitously expressed throughout the CNS and when activated, positively regulates neuroinflammatory genes in glia and induces prosurvival/synaptic plasticity genes in neurons. Therefore, targeting cell-specific NF- kappaB is critical for a desirable outcome when intervening with PD pathology. In the present studies, we utilized several experimental strategies to target neuroinflammation in multiple animal models of parkinsonism. The first utilized an astrocyte-specific NF- kappaB knock-out (KO) mouse model. We showed that KO animals were protected from neurotoxin-induced PD pathology and associated behavior, thus supporting the importance of astrocyte- NF- kappaB activation in parkinsonism. The second experimental strategy utilized small molecular compound, C-DIM12, which has agonistic properties with orphan nuclear receptor Nurr1 (NR4A2). Nurr1 activation indirectly suppresses NF- kappaB regulated gene expression in glial cells and also positively regulates genes associated with the production/release of DA in neurons. Through counter-regulation of NF- kappaB by Nurr1 activation with orally delivered C-DIM12, a neuroprotective effect was conveyed to the SN in a similar experimental mouse model of PD. Lastly, an alternative approach to modulating neuroinflammation utilized adeno-associated viruses (AAVs) to target specific cells of the CNS for transgene expression. Data revealed multiple AAV serotypes differed in transduction capacity and by comparison, an optimal serotype was identified for astrocyte targeting. Using AAV technology, transgene Nurr1 was overexpressed in astrocytes in vitro and in vivo. The preliminary results with AAV-Nurr1 display a protective effect against neurotoxicity and a promising direction for future studies. In conclusion, these studies investigate the role of neuroinflammation in relation to DA neuronal injury and provide disease modification strategies by cell-specifically targeting NF- kappaB and Nurr1 signaling pathways.
Notlar:
School code: 0053
Tüzel Kişi Ek Girişi:
Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(678690.1) | 678690-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
On Order
Liste seç
Bunu varsayılan liste yap.
Öğeler başarıyla eklendi
Öğeler eklenirken hata oldu. Lütfen tekrar deneyiniz.
:
Select An Item
Data usage warning: You will receive one text message for each title you selected.
Standard text messaging rates apply.