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Molecular analysis of a tsetse gut protein with putative agglutinating activity
Başlık:
Molecular analysis of a tsetse gut protein with putative agglutinating activity
Yazar:
Chandra, Manosree, author.
ISBN:
9780438057166
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (262 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Advisors: Dave Barry.
Özet:
Tsetse flies (Glossina) are vectors of trypanosomes, the causative agent of human and animal African trypanosomiasis. Trypanosoma brucei, which includes subspecies pathogenic to humans, has a complex life cycle alternating between mammal and fly; the portion of its life cycle in the fly has two phases, establishment of infection in the gut, and in the salivary gland. Tsetse lectins are thought to influence the establishment of trypanosomal infection in both the gut and salivary glands. A protein isolated in this laboratory (named pld37) was tentatively identified as a lectin with some features in common with a gut activity thought to affect trypanosome establishment in the gut. This project was undertaken with the aim of characterising both the genomic sequence of the lectin and its agglutinating activity. Three different cDNA clones which corresponded to the deduced pld37 DNA sequence were isolated by PCR. A notable characteristic of all three clones was the presence of about 100 proline/glutamate repeats, the repeats also being seen in the major surface protein, PARP, of the trypanosome procyclic stage in the fly gut. Recombinant proteins containing the full length and partial sequence were generated, and mouse antisera produced against the latter. Fly extract, recombinant protein and isolated pld37 preparations were probed using an anti-glutamate-proline monoclonal antibody that had been raised against PARP. Initial agglutinating assays using isolated pld37 and gut extracts from G m morsitans and G m centralis proved inconclusive, but an improved protocol yielded definitive results. Further work is required to characterise the expression and agglutinating activity of the pld37, a protein which has potential to be incorporated into a disease control strategy against African trypanosomiasis.
Notlar:
School code: 0547
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684575.1) | 684575-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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