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Demonstration of viruses in tissues
Başlık:
Demonstration of viruses in tissues
Yazar:
Turbitt, Marlyn L., author.
ISBN:
9780438060104
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (265 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Advisors: R. B. Goudie.
Özet:
This thesis records attempts to demonstrate viral antigens in order to detect infection by hepatitis B virus which cannot be cultured in vitro. It was hoped that viral antigen would be found more frequently in the liver tissue where the virus replicates, than in serum which is normally tested for hepatitis B viral antigens because it is readily available, A radioimmunoassay test was used to look for hepatitis B surface antigen in liver and blood samples obtained at post-mortem. Positive results were found no more often in liver than in serum from patients dying in Glasgow and in Nairobi, a finding which might be partly explained by enzymatic destruction of HBsAg in post-mortem liver homogenates kept at room temperature or above. Baby mice were infected with Coxsackie virus B1 as a model system to demonstrate viral antigens in tissue by immunohistochemical techniques. Animals were successfully infected with the virus as shown by characteristic electron microscopic lesions and viral antigens were demonstrated in the pancreas by immunohistochemical staining at light microscopic level. Hepatitis B surface antigen in paraffin sections of liver was studied by the immunoperoxidase technique. It was found necessary to confirm the specificity of the staining by blocking the antiserum with HBsAg. Immunoperoxidase staining of HBsAg in paraffin sections of liver was shown not to be subtype specific. A novel method was used to determine the sensitivity of the immunoperoxidase technique by staining paraffin sections containing dilutions of HBsAg rich serum. It was shown that radioimmunoassay is probably a more sensitive technique for detecting small amounts of antigen. The immunoperoxidase technique for staining HBsAg in paraffin sections of liver enabled a retrospective epidemiological survey to be carried out. Cases of primary hepatocellular carcinoma, cirrhosis and normal controls from both Scotland and Africa and alcoholic cirrhosis in Scotland were stained for HBsAg in an attempt to find an association between hepatitis B virus infection and these liver diseases, A significant increase in the incidence of HBsAg was found in cirrhosis and hepatocellular carcinoma in both Scotland and Africa, A possible interpretation of these findings is that hepatitis B virus may be one of many possible factors in the development of cirrhosis and hepatocellular carcinoma. The result of the survey of alcoholics was equivocal but the possibility of the involvement of hepatitis B virus in alcoholic liver disease cannot be ruled out without further investigation. Hepatitis B surface antigen was looked for in the immunoperoxidase technique in tissues other than liver from infected individuals but no staining was found suggesting that hepatitis B virus replicates only in the liver.
Notlar:
School code: 0547
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684787.1) | 684787-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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