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Immunological Characterization of Malaria Glutamic Acid-rich Protein (GARP)
Başlık:
Immunological Characterization of Malaria Glutamic Acid-rich Protein (GARP)
Yazar:
Kaiser, Maima Molvina, author.
ISBN:
9780438031180
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (54 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Athar H. Chishti Committee members: Miguel Stadecker.
Özet:
Malaria is the most predominant parasitic infection and continues to have a significant global impact on the health and well-being of hundreds of millions of people annually [1]. Plasmodium falciparum Glutamic acid-rich protein(Pf-GARP) is a parasite-derived protein secreted during the blood stage of malaria. From the studies with field isolates, it appears that Pf-GARP is very immunogenic, and it is abundant in children from endemic areas [2, 3]. A key tool for the control, elimination, or even possible eradication of malaria, in addition to antimalarial drugs and vector control, is an effective vaccine[4]. My research focuses on the immunological characterization of Pf-GARP. These studies include the characterization of a new monoclonal antibody for Pf-GARP, mapping of the epitope of Pf-GARP by utilizing synthetic peptides, and initial localization of Pf-GARP using the GM7 monoclonal antibody (mAb) by immunofluorescence microscopy. Finally, to validate the proof of concept of its clinical significance, we developed an Enzyme-Linked Immunosorbent Assay (ELISA) to detect antibodies against Pf-GARP in the human malaria plasma. Our screens utilized recombinant His-Pf-GARP protein as well as defined synthetic peptides of Pf-GARP to detect and quantify antibodies in human plasma from malaria-endemic areas in Africa. In summary, we have characterized a new mAb, termed GM7, that is specific for Pf-GARP protein and determined a peptide (M1P6) that is the target of the binding of the antibody (Ab) against the Pf-GARP protein. These findings are likely to have implications for screening malaria patients that are seropositive for Pf-GARP, and its functional significance in the pathogenesis of cerebral and pregnancy-associated malaria.
Notlar:
School code: 0845
Konu Başlığı:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(689050.1) | 689050-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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