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Optical fiber tools for single cell trapping and manipulation
Başlık:
Optical fiber tools for single cell trapping and manipulation
Yazar:
Ribeiro, Ana Rita da Silva Rodrigues, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (201 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-09C.
Özet:
Through over 47 years of existence, the field of optical trapping has found no restrictions in extending across natural sciences, while fomenting cutting edge research. Currently, optical trapping setups rely on spacious and expensive microscopes, adapted to accommodate extra optical components to produce the desired optical trapping fields. Nevertheless, optical fibers are an attractive replacement offering compact and low cost solutions. Scaling down optical manipulation implicates transforming the optical fiber tips into special micro structures enabling the desired trapping by suitable intensity patterns (TIP). Therefore, this thesis draws on the development of optical fibers as platforms to produce adequate TIPs for manipulation of cells and micro particles.
In the first place, an overview of the state of the art of optical fiber TIPs is described, revealing the trending fabrication methods, most relevant findings and existing gaps in the literature. This leads to the following chapter, which presents some tools and techniques used throughout this thesis, that includes the experimental fabrication methods, a computational tool, based on finite difference time domain, to investigate the resulting optical beams and optical forces, and finally the methodology used to compute the optical forces.
Photo-polymerization of micro structures is presented at this point. This technique allows the fast production of low cost optical fiber TIPs. First, some preliminary experimental and computational results are shown regarding the micro structures designs and the materials used. Then, the polymeric optical fiber TIPs are characterized and validated by trapping yeast cells and organelles of plant cells.
In addition to the previous method, this thesis reports the fabrication of optical fiber TIPs by focused ion beam milling. Through this technique it is possible to carve micro structures with sub-wavelength resolution on optical fiber tips. First off, the potential of Fresnel zone and phase plates is explored. This covers computational studies, experimental beam profile measurements and implementation of phase plates for trapping and size-based detection of PMMA particles and yeast cells. With this technique also spiral phase plates were etched on single mode optical fibers, shaping the fundamental Gaussian mode into a Laguerre-Gaussian beam. Since these beams carry orbital angular momentum, not only trapping but also rotation of polystyrene particles and microalgae were observed using an optical fiber with a spiral phase plate.
In general, the reported optical fiber configurations convey novel practical developments to the optical trapping area. In particular, at INESC TEC, these optical fiber TIPs will be the groundwork for further improvements towards biomedical studies.
Notlar:
School code: 5896
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(691189.1) | 691189-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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