Eylem Seç
Biophysical Approaches For Studying Desolvation Energy In Dna:DNA Binding Systems
Başlık:
Biophysical Approaches For Studying Desolvation Energy In Dna:DNA Binding Systems
Yazar:
Harmon, Caroline Rose, author.
ISBN:
9780438117846
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (63 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Daryl K. Eggers Committee members: Laura C. Miller Conrad; Alberto A. Rascón, Jr.
Özet:
Hydration spheres have unique water behavior compared to the disorder of the bulk water phase. These hydration spheres have been shown to be crucial for structural and conformational stability, yet their effect on binding association has been largely mistreated. For a one-to-one binding system, we propose to append the classical Gibbs-free energy equation as follows: Delta G0 = --RT ln (Ki ) -- [AB]DeltaGiH2O). Having a stronger grasp on how desolvation energy affects binding will ultimately aid studies from drug development and metabolism, to interpretation of structural models. To test our equation, we studied two small oligonucleotides that follow Watson-Crick base pairing using two different methods. We used (1) Isothermal Titration Calorimetry (ITC), a well-established binding method that measures the heat from direct binding interactions as one reactant is titrated into the system, and (2) Microscale Thermophoresis (MST), a new method that directly measures the movement of a fluorescently-labeled molecule as heat is applied. In each case, as the concentration of bound DNA increased, we found the binding affinity weakened linearly in terms of the quantity --RTln(K), with R 2 > 0.90 and R 2 > 0.80 for ITC and MST, respectively. Thus, the desolvation energy had an unfavorable impact on DNA:DNA binding, and, interestingly, this energy is orders of magnitude larger than that of our published model system, chelation of calcium(II) by EDTA. The larger energy value for DNA:DNA binding is not too surprising given (a) the much larger surface area of the DNA binding interface relative to EDTA and calcium, and (b) recent reports of a water spine in the minor groove of the DNA double helix.
Notlar:
School code: 6265
Konu Başlığı:
Tüzel Kişi Ek Girişi:
Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(692994.1) | 692994-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
On Order
Liste seç
Bunu varsayılan liste yap.
Öğeler başarıyla eklendi
Öğeler eklenirken hata oldu. Lütfen tekrar deneyiniz.
:
Select An Item
Data usage warning: You will receive one text message for each title you selected.
Standard text messaging rates apply.