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New Paradigms in Paper-Based Microfluidics and Their Use in the Study of Materials and Their Applications
Başlık:
New Paradigms in Paper-Based Microfluidics and Their Use in the Study of Materials and Their Applications
Yazar:
Ilacas, Grenalynn Casel, author.
ISBN:
9780438071339
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (97 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Frank A. Gomez Committee members: Oscar Bernal; Michael L. Hayes; Yangyang Liu; Alison McCurdy.
Özet:
Microfluidic paper-based analytical devices (muPADs) have emerged as a promising platform for a variety of point-of-care (POC) applications especially in regions of limited resources owing to their reduced reagent consumption rate and short sampling-to-result time. muPADs are easily fabricated by patterning hydrophobic materials in hydrophilic paper. They are low cost, compatible with biological samples, easy to stack, store, transport, and wick aqueous fluids without the use of active pumping. Herein, three projects describing novel applications utilizing muPADs for their use in the study of materials and their applications are described.
For the first project, chemotactic behavior on a muPAD using as a model enzyme-substrate system glucose oxidase (GOx) and glucose is detailed. Chemotaxis is the movement of materials, particularly biological species, in response to the influence of chemical stimulation. Here, for the first time, chemotactic behavior on a microfluidic paper-based analytical device (muPAD) to afford a distribution of products not obtainable under other (non-muPAD) experimental conditions using as a model enzyme-substrate system glucose oxidase (GOx) and glucose.
The second project describes an application that involves enzyme stability by storage on muPAD chips as a dehydrated preparation for a POC glucose diagnostic device as well as miniaturization studies providing further device optimization. POC glucose diagnostic devices were stored under various temperature environments (-20 °C, 0 °C, and 20 °C) and results produced 3D surface plots of the enzyme stability relative to time and storage conditions. Accuracy of enzyme storage varied between the three temperature conditions, where calculated predictions of glucose detection was compared to a 7-day storage study with an accuracy range of percent errors from 0.89% -- 15.76%. The project also describes the miniaturization of the POC diagnostic devices using model substrate-enzyme assays detecting glucose and acetylthiocholine iodide (ATC). Results showed an increase of standard deviation as the devices decreased in size, however linear trends correlating an increase of glucose with an increase of color intensity was still observed for all miniaturization studies.
Finally, in the third project, a well-based microfluidic paper-based analytical device (muPAD) to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Fe-PCN-222, to encapsulate glucose oxidase (GOx) is described. The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx-MOF species sandwiched between the paper layers realizing a yellow-brown color. The LFA platform consisted of chromatography paper between parafilm and polyvinyl acetate (PVA) layers. MOFs spotted on the paper subjected to solutions of KI and glucose yielded a brown color. The devices were then dried, scanned, and analyzed yielding a correlation between yellow intensity and glucose concentrations. The use of the non-porphryrinic iron-based MOF, MIL-53(Fe) which can be substituted for a heme-peroxidase or porphyrin ring in a solution-phase environment was also examined.
Notlar:
School code: 0962
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(694093.1) | 694093-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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