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Plasma protein binding of on123300 in rat plasma using rapid equilibrium dialysis
Başlık:
Plasma protein binding of on123300 in rat plasma using rapid equilibrium dialysis
Yazar:
Tembre, Neha Sudhir, author.
ISBN:
9780438079151
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (64 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: David Taft.
Özet:
ON123300 is a multi-targeted kinase inhibitor which has selective CDK4 and CDK6 inhibitor and anticancer activity. The purpose of the thesis is to validate HPLC method for ON123300 and to determine the plasma protein binding of ON123300 in rat plasma using rapid equilibrium dialysis device technique. HPLC method was validated with mobile phase and with plasma using concentration range (0.05.. 0.5. 1, 2.5, 5, 7.5, 10 mug/mL) and (0.25. 0.5. 1, 2.5. 5, 7.5, 10 mug/mL) respectively. Deionized water and acetonitrile 60:40 proportion with phosphate buffered saline (p11 7.2) was used as a mobile phase with flow rate 1.0 ml/min and run time 12 min. The detection wavelength used for ON123300 was 223nm and the mobile phase Separation was achieved by using a reverse phase analytical column (Agilent Zorbax Eclipsed XBD-C18 column, 4.6x 150 mm.. 5um. Agilent technologies, USA). The plasma binding experiments were carried at concentration at (5,7.5, 10 mug/mL) and fraction unbound was determined by using rapid equilibrium dialysis technique. ON123300 was found to be highly plasma protein bound drug (~99%). This corresponds to an unbound fraction in plasma (fu) less than 0.01 due to which there is very less free drug is available for pharmacological activity. Plasma binding is an important physiologic determinant of drug binding and clearance. Ongoing research is being carried out to characterize the pharmacokinetics of ON123300 in vivo in rats. As Plasma protein binding is very important aspect when it conies to determine dosage regimen for any drug. plasma binding data and pharmacokinetic parameters such as clearance will provide useful information which is helpful in selection of dosage regimen for ON123300.
Notlar:
School code: 0198
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(696258.1) | 696258-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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