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The Contact Mechanics of Soft Surfaces: Cells, Gels and Elastomers
Başlık:
The Contact Mechanics of Soft Surfaces: Cells, Gels and Elastomers
Yazar:
Schulze, Kyle Dwight, author.
ISBN:
9780438122130
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (88 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Advisors: W. Gregory Sawyer.
Özet:
Mechanical indentation tests are performed to understand the elastic properties of three ubiquitous soft, biphasic materials: PDMS silicone, PAAm hydrogels, and MDCK cell monolayers. PDMS silicone is shown to have strong hysteresis response to variation over a range of moduli, indentation rates, and indenter tip radii while being largely insensitive to indentation depth. No variable was seen to solely govern hysteresis or eliminate it, however decreasing indentation rate is critical to minimizing hysteresis. Hertzian elastic response of relatively thick PAAm hydrogel sheets was verified under short time scales under indentation while slow diffusion and polymer rearrangement were observed in longtime scales. Direct observation of the hydrogel surface under load, as well as bulk compression experiments of a hydrogel between two roughened plates, found that draining, and by extension compression of the hydrogel, can only occur if the applied pressure exceeds the osmotic pressure of the gel. Gentle indentation of MDCK monolayers demonstrate they will respond to load initially in accordance with the Winkler Foundation model. Over these short timescales a compressive modulus commensurate with moduli values found with traditional cell mechanics measurements can be calculated for untreated cell monolayers. Relaxing of the cytoskeleton with a Myosin II inhibitor lowered the calculated cell layer moduli to 1/3 the untreated value suggesting the cytoskeletal pre-stress directly affects compressive modulus. Over long timescales the cells resistance to fluid transport was found to dominate their force-response to persistent loading.
Notlar:
School code: 0070
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(696642.1) | 696642-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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