Eylem Seç
The Impact of Mab21l2 Deletion on Neuronal Specification and other Neural Function
Başlık:
The Impact of Mab21l2 Deletion on Neuronal Specification and other Neural Function
Yazar:
Chan, Chung Man, author.
ISBN:
9780438159006
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (188 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Advisors: King Lau CHOW.
Özet:
The patterning of the vertebrate central nervous system is manifested as an organizedproduction of specific neuronal subtypes with cell fate specification being part of thisprocess. In this study, I focus on the role of an evolutionarily conserved gene, Mab21l2, in neural development. mab-21 was first identified in Caenorhabditis elegans as a cell fate specification gene in controlling male tail sensory ray development. Two vertebrate mab-21 homologs, Mab21l1 and Mab21l2, werereported to be highly expressed in partially overlapping domains in midbrain, eye,branchial arches, limb bud and spinal cord, while Mab21l2 was often expressed at ahigher level than Mab21l1. Mab21l2 is essential for embryo survival as null mutantof Mab21l2 dies at mid-gestation stage. In this study, I generated neural progenitorcell-specific knock-out mice (Mab2l12f/f;Nes-Cre) to investigate its role in neuraldevelopment.
Mab21l2 is highly expressed in multiple subtypes of postmitotic spinal interneuronsand various brainstem nuclei including those involved in respiration. Mab2l12f/f;Nes-Cre mutants appear morphologically normal at all embryonic stages. Abnormality of cellfate specification or neuronal patterning was not detected in mutant spinal cord. Atbirth, Mab2l12f/f;Nes-Cre mutants have a beating heart, but show no breathingmovements. They turn cyanotic and die within minutes after birth due to respiratoryfailure. Postmortem lung examination never revealed dilated lung alveoli in mutantneonates despite a normal pattern of diaphragmatic innervation and neuromuscularformation. The lethal phenotype is possibly due to defect in the central control ofrespiration. Impaired development of NK1R/SST co-expressing preBötC neurons butnot the RTN/pFRG neurons nor other respiratory motor nuclei in the medulla impliesthat Mab21l2 is critical for specifying the core rhythmogenic control, the deficit ofwhich contributes to the respiratory failure of the homozygous neonates.
Notlar:
School code: 1223
Konu Başlığı:
Tüzel Kişi Ek Girişi:
Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(697187.1) | 697187-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
On Order
Liste seç
Bunu varsayılan liste yap.
Öğeler başarıyla eklendi
Öğeler eklenirken hata oldu. Lütfen tekrar deneyiniz.
:
Select An Item
Data usage warning: You will receive one text message for each title you selected.
Standard text messaging rates apply.